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KMID : 1234420090370020153
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Korean Journal of Microbiololgy and Biotechnology
2009 Volume.37 No. 2 p.153 ~ p.159
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Optimizing the Production of 5-Aminolevulinic Acid by Recombinant Escherichia coli Containing the Rhodobacter capsulatus hemA Gene
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Yang Dong-Soo
Park Moon-Won
Lim Soo-Jin
Kim MIn-Jeong
Shin Yu-Ri
Park Chan-Soo
Hyun Young
Kang Dae-Kyung
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Abstract
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Recombinant Escherichia coli BLR(DE3) harboring the hemA gene from Rhodobacter capsulatus under the control of a constitutive promoter, which we constructed previously, was used for the extracellular production of 5-aminolevulinic acid (ALA). The effects of several factors on ALA production were investigated in flask culture. ALA production by the recombinant E. coli was more efficient at 30oC than 37oC. The glycine concentration had an important effect on cell growth. Glycine and succinic acid concentration of 5-10 and 10-20 g/L, respectively, resulted in high ALA production. In addition, the partial replacement of succinic acid by sodium glutamate increased the ALA production. The ALA production was inhibited by the presence of glucose in the medium. Using the optimal conditions, an ALA concentration of 8.2 g/L was achieved in jar fermentation without an added inducer or ALA dehydratase inhibitor; this is the highest reported concentration.
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KEYWORD
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5-Aminolevulinic acid, recombinant Escherichia coli, glycine, succinic acid, glutamate, glucose
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